Βούλα Βελισσαρίου ΒSc, PhD

Επιστημονική Διευθύντρια

Τμήμα Γενετικής και Μοριακής Βιολογίας Ομίλου ΒΙΟΙΑΤΡΙΚΗ

H Δρ Βούλα Βελισσαρίου είναι Επιστημονική Διευθύντρια του Τμήματος Γενετικής και Μοριακής Βιολογίας και μέλος του Επιστημονικού Συμβουλίου στον Όμιλο ΒΙΟΙΑΤΡΙΚΗ.

Έχει πτυχίο Βιολογίας από το ΕΚΠΑ και διδακτορικό δίπλωμα (Phd) στη Γενετική από το Πανεπιστήμιο του Cambridge. Υπήρξε μεταδιδακτορική ερευνήτρια με υποτροφία στο Πανεπιστήμιο Cambridge, με εξειδίκευση στην Κυτταρογενετική. Στη συνέχεια εξειδικεύθηκε στην Κλινική Κυτταρογενετική σε πανεπιστημιακά νοσοκομεία στην Οξφόρδη και στο Λονδίνο. 

Εργάσθηκε στο δημόσιο τομέα στα νοσοκομεία Αγία Σοφία και Αλεξάνδρα στον τομέα της προγεννητικής και μεταγεννητικής διάγνωσης των χρωμοσωματικών ανωμαλιών. Το 1998 ίδρυσε το τμήμα Γενετικής και Μοριακής Βιολογίας στο νοσοκομείο Μητέρα του ομίλου Υγεία, στο οποίο ήταν διευθύντρια επί 17 έτη.  Από το 2013 έως το 2015 ήταν διευθύντρια του εργαστηρίου Κυτταρογενετικής στο Alab του Ομίλου Υγεία.  

Είναι μέλος της επιστημονικής ομάδας η οποία ανέπτυξε μία από τις πλέον αξιόπιστες εξετάσεις διεθνώς μη επεμβατικού προγεννητικού ελέγχου σε ελεύθερο εμβρυϊκό DNA στο αίμα της εγκύου με συμμετοχή σε πολλές διεθνείς δημοσιεύσεις. Επίσης, είναι αξιολογητής του Ε.Σ.Υ.Δ (Εθνικό Σύστημα Διαπίστευσης) κλινικών εργαστηρίων γενετικής διάγνωσης χρωμοσωματικών ανωμαλιών. Υπήρξε ιδρυτικό και εκλεγμένο μέλος του ΔΣ του Σ.Ι.Γ.Ε. (Σύνδεσμος Ιατρικών Γενετιστών Ελλάδος) και της ΠΕΒ (Πανελλήνιας Ένωσης Βιοεπιστημόνων) και μέλος του επιστημονικού συμβουλίου του νοσοκομείου Μητέρα. Είναι εκλεγμένη εκπρόσωπος του Σ.Ι.Γ.Ε. στην ECA (European Cytogeneticists Association). Συμμετέχει στην επιτροπή του Σ.Ι.Γ.Ε. στην οποία το Υπουργείο Υγείας έχει αναθέσει την ειδικότητα της Εργαστηριακής Γενετικής.

Είναι συγγραφέας πολλών δημοσιεύσεων στον τομέα της προγεννητικής και μεταγεννητικής γενετικής διάγνωσης και κριτής άρθρων προς δημοσίευση σε έγκριτα επιστημονικά περιοδικά του χώρου. Έχει, επίσης, πλούσια διδακτική εμπειρία στον τομέα της. 

Ενδεικτικές δημοσιεύσεις

  • Velissariou V, Ashburner M. Τhe Secretory Proteins of the Larval Salivary Gland of Drosophila Melanogaster: Cytogenetic Correlation of a Protein and a Puff.  1980. Chromosoma (Berl.) 77: 13-27.
  • Velissariou V, Ashburner M. Cytogenetic and Genetic Mapping of a Salivary Gland Secretion Protein in Drosophila Melanogaster. 1981. Chromosoma (Berl.) 84:173-185.
  • Pangalos C, Velissariou V, Liacacos G. Ring-14 and Trisomy 14q in the Same Child. 1984. Ann. Genet. 27:28-40.
  • Tsita K.P, Vallas O.S, Velissariou V. J, Lyberatou-Moraitou E.K. Α case of prenatal diagnosis of a familiar satellited Yq chromosome. 1989. Clinical Genetics 35: 70-74.
  • Lyberatou E, Velissariou V. Single cell structural abnormalities in couples with habitual abortions. 1991.  Iatriki 60:273-275.
  • Lyberatou E, Velissariou V, Kreatsas G. 1991.Primary amenorrhea in a girl with Xq-. Iatriki 60:498-500. 
  • Velissariou V, Lyberatou E, Antonopoulou E, Polymilis C. “Chromosome Breakage in Individuals with Single-Cell Structural Aberrations and Habitual Abortions.1993. Gynec Obstet Invest 36:71-74. 
  • Velissariou V, Lyberatou E, Grigori P, Kosmaidou Z, Mesogitis S, Antsaklis A.  1993. Chromosome Abnormalities Detected in Dysmorphic Fetuses During Routine Prenatal Diagnosis and in Pregnancies with Abnormal Ultrasound Findings: Α study of 3128 Cases. Dysmorphology and Genetics of Cardiovascular Disorders.  Eds C. Bartsokas and Peter Beighton.
  • Lyberatou E, Velissariou V, Kosmaidou Z, Grigori P, Tsita K, Valla O, Kammenou Z, Antsaklis A, Mesogitis S, Aravantinos D. 1993. Fetal blood chromosomal analysis as a method of prenatal diagnosis. Iatriki 63:581-586. 
  • Syrrou M, Velissariou V, Lyberatou E, Pagoulatos G. 1996. Application of fluorescence in situ hybridization (FISH) in three cases of patients with chromosomal abnormalities. Iatriki 70:69-72.
Περισσότερα
  • Velissariou V, Grigori P, Agapitos M, Alexandrakis G, Lyberatou E. Holoprosencephaly in a fetus with de novo terminal deletion of the long arm of chromosome 7, del(7)(q32). 1997. Iatriki 71:175-177.
  • Rizos D, Sarandakou A, Velissariou V, Liberatou E, Hassiakos D, Pirgiotis E, Phocas I. The Influence of hCG and uE3 Population Statistical Parameters on Biochemical Screening for Chromosomal Anomalies in the Second Trimester of Pregnancy. 1996. Chimika Chronika, New Series 25:134.
  • Marcoulatos P, Koussidis G, Mamuris Z, Velissariou V, Vamvakopoulos N.  “Mapping Interleukin Enhancer Binding Factor 2 Gene (ILF2) to Human Chromosome 1 (1q11-qter and 1p11-p12) by Polymerase Chain Reaction Amplification of Human-Rodent Somatic Cell Hybrid DNA Templates”. 1996. J Interferon Cytokine Res 16:1035-1038.
  • Patsalis PC, Hadjimarcou MI , Velissariou V , Kitsiou-Tzeli S, Lyberatou E, Skordis N. “Supernumerary marker chromosomes (SMCs) in Turner syndrome are mostly derived from the Y chromosome”. 1997. Clin Genet 51:184-190.
  • Rizos D, Velissariou V, Phoka I, Sarandakou A, Lyberatou E, Kassanos D, Hasiakos D, Botsis D, Chrisikopoulos A. Biochemical  prenatal screening for Down’s syndrome in women older than 35 years with the double or triple test. 1997. Hellen Obstet Gynecol 9:128-136.
  • Velissariou V, Andoniadi T, Patsalis P, Hajipouliou A, Christopoulou S, Donoghue J, Bakou K, Kaminoretros P, Athanassiou V, Petersen MB. Two rare de novo structural aberrations of the Y chromosome: cytogenetic and molecular analysis during prenatal diagnosis. 2001.  Prenat Diagn 21:484-487.
  • Velissariou V, Andoniadi T, Gyftodimou J, Bakou K, Grigoriadou M, Christopoulou S, Hatzipouliou A, Donoghue J, Karatzis P, Katsarou E, Petersen MB. Maternal uniparental disomy 20 in a fetus with trisomy 20 mosaicism: clinical, cytogenetic and molecular analysis. 2002. Eur J Hum Genet 10:694-698. 
  • Antoniadi T, Yiapitjakis C,  Kaminopetros P, Makatsoris C, Velissariou V, Vassilopoulos D, Petersen MB. A simple and effective approach for detecting maternal cell contamination in molecular prenatal diagnosis. 2002. Prenat Diagn 22:425-429.
  • Velissariou V.  Uniparental Disomy (UPD): A consequence of non-disjunction and the implications in prenatal diagnosis. 2003. BJMG 6:55-59.
  • Sifakis S, Velissariou V, Papadopoulou E, Petersen MB, Koumantakis E. Prenatal Diagnosis of Trisomy 2 Mosaicism: A Case Report. 2004. Fetal Diagn Ther 19:488-490.
  • Mihalatos M, Apessos A, Douwerse H, Velissariou V, Psychias A, Koliopanos, Petropoulos K, Triantafillidis JK, Danielidis I, Foutzilas G, Agnantis NJ, Nasioulas G. Rare mutations predisposing to familial adenomatous polyposis in Greek FAP patients. 2005. BMC Cancer 5:40.
  • Karadimas C, Sifakis S, Valsamopoulos P, Makatsoris C, Velissariou V, Nasioulas G, Petersen MB, Koumantakis E, Hatzaki A. Prenatal Diagnosis of Hypochondroaplasia: Report of Two Cases. 2006.  AJMG
  • Velissariou V, Christopoulou S, Karadimas C, Pihos I, Kanaka -Gantenbein C, Kapranos N, Kallipolitis G, Hatzaki A. Rare XXY/XX mosaicism in a phenotypic male with Klinefelter syndrome: Case report. 2006. EJMG 49:331-337.
  • Karadimas C, Trouvas D, Haritatos G, Makatsoris C, Dedoulis E, Velissariou V, Antoniadi T, Hatzaki A, Petersen MB. Prenatal diagnosis of achondroplasia presenting with multiple-suture synostosis: a novel association. 2006. Prenat Diagn 26(3):258-261.
  • Velissariou V, Sismani C, Christopoulou S, Kaminopetros P, Hatzaki A, Evangelidou, Koumbaris G, Bartsokas CS, Stylianidou G, Skordis N, Diakoumakos, Patsalis PC. Loss of the Y chromosome PAR2 region and additional rearrangements in two familial cases of satellited Y chromosomes: Cytogenetic and molecular analysis. 2007. Eur J Med Genet 50:291-300.
  • Kitsiou-Tzeli S, Sismani C, Karkaletsi M, Florentin L, Anastassiou A, Koumbaris G, Evangelidou P, Agapitos E, Patsalis P, Velissariou V. Prenatal diagnosis of a de novo partial trisomy 10p12.1-12.2 pter originating from an unbalanced translocation onto 15qter and confirmed with array CGH. 2008. Prenat Diagn 28:770-772.
  • Sifakis S, Karkaletsi M, Christopoulou S, Donoghue J, Kaminopetros P, Konstantinidou T, Velissariou V. Distinctive pattern of first trimester maternal serum biochemical markers in trisomy 22 pregnancies. 2008. Prenat Diagn 28:1174-1176.
  • Sifakis S, Koukoura O, Mantas N, Velissariou V, Koumantakis E. Hydrops fetalis, thickened placenta, and other sonographic findings in a low-level trisomy 21 mosaicism: a case report. 2008. Fetal Diagn Ther 24:310-312.
  • Konstantinidou AE, Agrogiannis G, Sifakis S, Karantanas A, Harakoglou V, Kaminopetros P, Hatzaki A, Petersen MB, Karadimas C, Velissariou V, Velonis S, Papantoniou N, Antsaklis A, Patsouris E. Genetic skeletal disorders of the fetus and infant: pathologic and molecular findings in a series of 41 cases. 2009. Birth Defects Res A Clin Mol Teratol 85:811-821.
  • Christopoulou S, Christopoulou G, Hatzaki A, Hatzipouliou A, Donoghue J, Karkaletsi M, Kaminopetros P, Sifakis S, Velissariou V. The replacement of cytogenetic analysis by direct chorionic villi sampling preparation with quantitative fluorescence PCR. 2009. Gynecol Obstet Invest 68:255-261.
  • Sifakis S, Staboulidou I, Maiz N, Velissariou V, Nicolaides KH. Outcome of pregnancies with trisomy 2 cells in chorionic villi. 2010. Prenat Diagn 30:329-332.
  • Chrissouli S, Pratsinis H, Velissariou V, K Letsas D. Human amniotic fluid stimulates the proliferation of human fetal and adult skin fibroblasts: The roles of bFGF and PDGF and the ERK and Akt signaling pathways. 2010. Wound Repair Regen. 18(6):643-54.
  • Evangelidou P, Sismani C, Ioannides M, Christodoulou C, Koumbaris G, Kallikas I, Georgiou I, Velissariou V, Patsalis PC. 2010. Clinical application of whole-genome array CGH during prenatal diagnosis: Study of 25 selected pregnancies with abnormal ultrasound findings or apparently balanced structural aberrations. Mol Cytogenet. 26;3:24. PMID:21110858.
  • Papageorgiou EA, Karagrigoriou A, Tsaliki E, Velissariou V, Carter NP, Patsalis PC. 2011. Fetal- specific DNA methylation ratio permits noninvasive prenatal diagnosis of trisomy 21. Nat Med. 17(4):510-3.
  • Christopoulou G, Tzetis M, Konstantinidou AE, Tsezou A, Kanavakis E, Kitsiou-Tzelli S, Velissariou V. 2011. 12.59 Mb and 4.22 Mb deletions in chromosomal bands 10p15.3→p14 and 10q26.3, respectively, in a fetus with talipes equinovarus, macrocephaly, stubby nose, hypertelorism, micrognathia and syndactyly of both hands and feet. 2012. Eur J Med Genet. 55(1):75-9.
  • Tsaliki E, Papageorgiou EA,…Velissariou V, Patsalis PC. 2012. MeDIP real-time qPCR of maternal peripheral blood releiably identifies trisomy 21. 2012. Prenat Diagn. 32(10): 996-1001.
  • Patsalis PC, Tsaliki E, Koumbaris G, Karagrigoriou A, Velissariou V, Papageorgiou EA. 2012. A new non-invasive prenatal diagnosis of Down syndrome through epigenetic markers and real-time qPCR. Expert Opin Biol Ther. 2012 Jun; 12 Suppl 1:S155-61. doi: 10.1517/14712598.2012.674108. Epub 2012 Apr 14.
  • Evangelidou P, Alexandrou A, Moutafi M, Ioannides M, Antoniou P, Koumbaris G, Kallikas I, Velissariou V, Sismani C and Patsalis P. Implementation of high resolution whole genome array CGH in the prenatal clinical setting. Advantages, challenges and review of the literature. 2013. Bio Med Research International. Volume 2013 (2013), Article ID 346762.
  • Kyriakou S, Kypri E, Spyrou C, Tsaliki E, Velissariou V, Papageorgiou EA, Patsalis PC.Variability of ffDNA in maternal plasma does not prevent correct classification of trisomy 21 using MeDIP qPCR methodology. 2013. Prenat Diagn. 33(7):650-5.
  • Christopoulou G, Sismani C, Sakellariou M, Saklamaki M, Athanassiou V, Velissariou V. Gene Clinical and molecular description of the prenatal diagnosis of a fetus with a maternally inherited microduplication 22q11.2 of 2.5Mb. 2013. Gene. Mar 16. Doi:10.1016
  • Sismani C, Donoghue J, Alexandrou A, Karkaletsi M, Christopoulou S, Konstantinidou AE, Livanos P, Patsalis PC, Velissariou V. A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus Gene. 2013 Aug 22. doi:pii: S0378-1119(13)01062-710.1016/j.gene.2013.08.032. [Epub ahead of print] 
  • Nazaryan L, Stefanou EG, Hansen C, Kosyakova N, Bak M, Sharkey FH, Mantziou T, Papanastasiou AD, Velissariou V, Liehr T, Syrrou M, Tommerup N. The strength of combined cytogenetic and mate-pair sequencing techniques illystrated by a germline chromothripsis rearrangement involving FOXP2. Eur J Hum Genet. 2013 Jul 17. doi: 10.1038/ejhg.2013.147. 
  • Patsalis PC, Christopoulou G, Velissariou V. Technical concerns on the clinical validation of the NIFTY test. Prenat Diagn. 2013 Apr 26. doi: 10.1002/pd.4146. [Epub ahead of print].
  • Konstantinidou AE, Tassoulas I, Kallipolitis G, Gasparatos S, Velissariou V, Paraskevakou H. Mandibulofacial dysostosis (Treacher-Collins syndrome) in the fetus: novel association with pectus carinatum in a molecularly confirmed case and review of the fetal phenotype. Birth Defects Res A Clin Mol Teratol 2103 Nov 29. doi: 10.1002/bdra.23202
  • Sismani C, Donoghue J, Alexandrou A, Karkaletsi M, Christopoulou S, Konstantinidou AE, Livanos P, Patsalis PC, Velissariou V. A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus. Gene. 2013 Nov 1; 530(1):138-42. doi: 10.1016/j.gene.
  • Sismani C, Christopoulou G, Alexandrou A, Evangelidou P, Donoghue J, Konstantinidou AE, Velissariou V. A Prenatally Ascertained De Novo Terminal Deletion of chromosomal bands 1q43q44 associated with multiple congenital    abnormalities in a female fetus. 2015. Case Reports in Genetics. Article ID 517678 6 pages http://dx.doi.org/10.1155/2015/517678.
  • Christopoulou G, Papageorgiou EA, Patsalis PC, Velissariou V. Review. Comparison of next generation sequencing-based and methylated DNA immunoprecipitation-based approaches for fetal aneuploidy non-invasive prenatal testing. 2015. World Journal Med Genet, Vol 5(2). DOI: 10.5496/wjmg
  • Velissariou V. and Patsalis P.C. Non-invasive prenatal testing for fetal aneuploidy in maternal blood. 2015. Molecular Diagnostics, Third Edition. Eds George P. Patrinos, Phillip B. Danielson, and Wilhelm J. Ansorge. Elsevier.
  • Koumbaris G, Kypri E, Tsangaras K, Achilleos A, Mina P, Neofytou M, Velissariou V, Christopoulou G, Kallikas I, Gonza A, Benusiene E, Latos-Bielenska A, Marek P, Santana A, Nagy N, Papageorgiou EA, Ioannides M, and Patsalis PC. 2016. Cell-Free DNA Analysis of Targeted Genomic Region in Maternal Plasma for Non-Invasive Prenatal Testing of Trisomy 21, Trisomy 18, Trisomy 13 and Fetal Sex. Clin Chemistry 62:6
  • Velissariou V and Patsalis PC. 2016. Non-invasive Cell- Free DNA Prenatal Testing for Fetal Aneuploidy in Maternal Blood. Molecular Diagnostics, Third Edition, Chapter 27. Elsevier. Eds.  Patrinos GP, Danielson P and Ansorge WJ. 
  • Roumelioti FM, Louizou E, Karras S, Neroutsou R, Velissariou V, Gagos S. Unbalanced X;9 translocation in an infertile male with de novo duplication Xp22.31p22.33. 2019. Journal of Assisted Reproduction and Genetics. https://doi.org/10.1007/s10815-019-01405-0
  • Kypri E, Ioannides M, Touvana E, Neophytou I, Mina P, Velissariou V, Vittas S, Santana A, Alexidis F, Tsangaras K, Achilleos A, Patsalis P and Koumbaris G. Non-invasive prenatal testing of fetal chromosomal aneuploidies: validation and clinical performance of the veracity test. 2019. Molecular Cytogenetics 12:34. https://doi.org/10.1186/s13039-019-0446-0 
  • Carolina Sismani, Stamatia-Maria Rapti, Pavlina Iliopoulou, Anastasia Spring, Rozalia Neroutsou, Magdalini Lagou, Marianna Robola, Efstathios Tsitsopoulos, Ludmila Kousoulidou, Angelos Alexandrou, Ioannis Papaevripidou, Athina Theodosiou, Maria Syrrou, Sigrid Fuchs, Maja Hempel, Dagmar Huhle, Thomas Liehr, Monika Ziegler, Max Duesberg, Voula Velissariou. 2020. Novel pericentric inversion inv(9)(p23q22.3) in unrelated individuals with fertility problems in the Southeast European population. Journal of Human Genetics https://doi.org/10.1038/s10038-020-0769-z. 
  • Velissariou V, Sachinidi F, Christopoulou S, Florentin L, Liehr T, Efthymiadou A, Angelopoulou E, Chrysis D, Stefanou EG. 2020. Low-Level Trisomy 14 Mosaicism: A Carrier of an Isochromosome 14 and a Supernumerary Marker Chromosome 14. Cytogenet Genome Res. Nov 17:1-7. doi: 10.1159/000511549.